diff --git a/config/set/php81.php b/config/set/php81.php
index 17cbdcee746..b87051387c8 100644
--- a/config/set/php81.php
+++ b/config/set/php81.php
@@ -8,6 +8,7 @@
 use Rector\Php81\Rector\Class_\SpatieEnumClassToEnumRector;
 use Rector\Php81\Rector\FuncCall\NullToStrictStringFuncCallArgRector;
 use Rector\Php81\Rector\MethodCall\MyCLabsMethodCallToEnumConstRector;
+use Rector\Php81\Rector\MethodCall\RemoveReflectionSetAccessibleCallsRector;
 use Rector\Php81\Rector\MethodCall\SpatieEnumMethodCallToEnumConstRector;
 use Rector\Php81\Rector\New_\MyCLabsConstructorCallToEnumFromRector;
 use Rector\Php81\Rector\Property\ReadOnlyPropertyRector;
@@ -24,5 +25,6 @@
         SpatieEnumMethodCallToEnumConstRector::class,
         NullToStrictStringFuncCallArgRector::class,
         FirstClassCallableRector::class,
+        RemoveReflectionSetAccessibleCallsRector::class,
     ]);
 };
diff --git a/rules-tests/Php81/Rector/MethodCall/RemoveReflectionSetAccessibleCallsRector/Fixture/fixture.php.inc b/rules-tests/Php81/Rector/MethodCall/RemoveReflectionSetAccessibleCallsRector/Fixture/fixture.php.inc
new file mode 100644
index 00000000000..c5ed1800193
--- /dev/null
+++ b/rules-tests/Php81/Rector/MethodCall/RemoveReflectionSetAccessibleCallsRector/Fixture/fixture.php.inc
@@ -0,0 +1,43 @@
+<?php
+
+namespace Rector\Tests\Php81\Rector\MethodCall\RemoveReflectionSetAccessibleCallsRector\Fixture;
+
+use ReflectionMethod;
+use ReflectionProperty;
+
+final class Fixture
+{
+    public function run(): void
+    {
+        $reflectionProperty = new ReflectionProperty($this, 'privateProperty');
+        $reflectionProperty->setAccessible(true);
+        $value = $reflectionProperty->getValue($this);
+
+        $reflectionMethod = new ReflectionMethod($this, 'privateMethod');
+        $reflectionMethod->setAccessible(false);
+        $reflectionMethod->invoke($this);
+    }
+}
+
+?>
+-----
+<?php
+
+namespace Rector\Tests\Php81\Rector\MethodCall\RemoveReflectionSetAccessibleCallsRector\Fixture;
+
+use ReflectionMethod;
+use ReflectionProperty;
+
+final class Fixture
+{
+    public function run(): void
+    {
+        $reflectionProperty = new ReflectionProperty($this, 'privateProperty');
+        $value = $reflectionProperty->getValue($this);
+
+        $reflectionMethod = new ReflectionMethod($this, 'privateMethod');
+        $reflectionMethod->invoke($this);
+    }
+}
+
+?>
diff --git a/rules-tests/Php81/Rector/MethodCall/RemoveReflectionSetAccessibleCallsRector/RemoveReflectionSetAccessibleCallsRectorTest.php b/rules-tests/Php81/Rector/MethodCall/RemoveReflectionSetAccessibleCallsRector/RemoveReflectionSetAccessibleCallsRectorTest.php
new file mode 100644
index 00000000000..b41e85eda5c
--- /dev/null
+++ b/rules-tests/Php81/Rector/MethodCall/RemoveReflectionSetAccessibleCallsRector/RemoveReflectionSetAccessibleCallsRectorTest.php
@@ -0,0 +1,31 @@
+<?php
+
+declare(strict_types=1);
+
+namespace Rector\Tests\Php81\Rector\MethodCall\RemoveReflectionSetAccessibleCallsRector;
+
+use Iterator;
+use PHPUnit\Framework\Attributes\DataProvider;
+use Rector\Testing\PHPUnit\AbstractRectorTestCase;
+
+final class RemoveReflectionSetAccessibleCallsRectorTest extends AbstractRectorTestCase
+{
+    #[DataProvider('provideData')]
+    public function testRule(string $filePath): void
+    {
+        $this->doTestFile($filePath);
+    }
+
+    /**
+     * @return Iterator<array<string>>
+     */
+    public static function provideData(): Iterator
+    {
+        return self::yieldFilesFromDirectory(__DIR__ . '/Fixture');
+    }
+
+    public function provideConfigFilePath(): string
+    {
+        return __DIR__ . '/config/configured_rule.php';
+    }
+}
diff --git a/rules-tests/Php81/Rector/MethodCall/RemoveReflectionSetAccessibleCallsRector/config/configured_rule.php b/rules-tests/Php81/Rector/MethodCall/RemoveReflectionSetAccessibleCallsRector/config/configured_rule.php
new file mode 100644
index 00000000000..c15b8139eea
--- /dev/null
+++ b/rules-tests/Php81/Rector/MethodCall/RemoveReflectionSetAccessibleCallsRector/config/configured_rule.php
@@ -0,0 +1,13 @@
+<?php
+
+declare(strict_types=1);
+
+use Rector\Config\RectorConfig;
+use Rector\Php81\Rector\MethodCall\RemoveReflectionSetAccessibleCallsRector;
+use Rector\ValueObject\PhpVersion;
+
+return static function (RectorConfig $rectorConfig): void {
+    $rectorConfig->rule(RemoveReflectionSetAccessibleCallsRector::class);
+
+    $rectorConfig->phpVersion(PhpVersion::PHP_85);
+};
diff --git a/rules/Php81/Rector/MethodCall/RemoveReflectionSetAccessibleCallsRector.php b/rules/Php81/Rector/MethodCall/RemoveReflectionSetAccessibleCallsRector.php
new file mode 100644
index 00000000000..a8d5e05abe4
--- /dev/null
+++ b/rules/Php81/Rector/MethodCall/RemoveReflectionSetAccessibleCallsRector.php
@@ -0,0 +1,88 @@
+<?php
+
+declare(strict_types=1);
+
+namespace Rector\Php81\Rector\MethodCall;
+
+use PhpParser\Node;
+use PhpParser\Node\Expr\MethodCall;
+use PhpParser\Node\Stmt\Expression;
+use PhpParser\NodeVisitor;
+use PHPStan\Type\ObjectType;
+use Rector\Rector\AbstractRector;
+use Rector\ValueObject\PhpVersion;
+use Rector\VersionBonding\Contract\MinPhpVersionInterface;
+use Symplify\RuleDocGenerator\ValueObject\CodeSample\CodeSample;
+use Symplify\RuleDocGenerator\ValueObject\RuleDefinition;
+
+/**
+ * As of PHP 8.1.0, calling `Reflection*::setAccessible()` has no effect.
+ *
+ * @see https://www.php.net/manual/en/reflectionmethod.setaccessible.php
+ * @see https://www.php.net/manual/en/reflectionproperty.setaccessible.php
+ * @see \Rector\Tests\Php81\Rector\MethodCall\RemoveReflectionSetAccessibleCallsRector\RemoveReflectionSetAccessibleCallsRectorTest
+ */
+final class RemoveReflectionSetAccessibleCallsRector extends AbstractRector implements MinPhpVersionInterface
+{
+    /**
+     * @return array<class-string<Node>>
+     */
+    public function getNodeTypes(): array
+    {
+        return [Expression::class];
+    }
+
+    /**
+     * @param Expression $node
+     */
+    public function refactor(Node $node): ?int
+    {
+        if ($node->expr instanceof MethodCall === false) {
+            return null;
+        }
+
+        $methodCall = $node->expr;
+
+        if ($this->isName($methodCall->name, 'setAccessible') === false) {
+            return null;
+        }
+
+        if ($this->isObjectType($methodCall->var, new ObjectType('ReflectionProperty'))
+            || $this->isObjectType($methodCall->var, new ObjectType('ReflectionMethod'))
+        ) {
+            return NodeVisitor::REMOVE_NODE;
+        }
+
+        return null;
+    }
+
+    public function getRuleDefinition(): RuleDefinition
+    {
+        return new RuleDefinition('Remove Reflection::setAccessible() calls', [
+            new CodeSample(
+                <<<'CODE_SAMPLE'
+$reflectionProperty = new ReflectionProperty($object, 'property');
+$reflectionProperty->setAccessible(true);
+$value = $reflectionProperty->getValue($object);
+
+$reflectionMethod = new ReflectionMethod($object, 'method');
+$reflectionMethod->setAccessible(false);
+$reflectionMethod->invoke($object);
+CODE_SAMPLE
+                ,
+                <<<'CODE_SAMPLE'
+$reflectionProperty = new ReflectionProperty($object, 'property');
+$value = $reflectionProperty->getValue($object);
+
+$reflectionMethod = new ReflectionMethod($object, 'method');
+$reflectionMethod->invoke($object);
+CODE_SAMPLE
+            ),
+        ]);
+    }
+
+    public function provideMinPhpVersion(): int
+    {
+        return PhpVersion::PHP_81;
+    }
+}
diff --git a/src/Util/Reflection/PrivatesAccessor.php b/src/Util/Reflection/PrivatesAccessor.php
index 0a67594c557..3d7512277ce 100644
--- a/src/Util/Reflection/PrivatesAccessor.php
+++ b/src/Util/Reflection/PrivatesAccessor.php
@@ -47,7 +47,6 @@ public function callPrivateMethod(object|string $object, string $methodName, arr
     public function getPrivateProperty(object $object, string $propertyName): mixed
     {
         $reflectionProperty = $this->resolvePropertyReflection($object, $propertyName);
-        $reflectionProperty->setAccessible(true);
 
         return $reflectionProperty->getValue($object);
     }
@@ -55,17 +54,13 @@ public function getPrivateProperty(object $object, string $propertyName): mixed
     public function setPrivateProperty(object $object, string $propertyName, mixed $value): void
     {
         $reflectionProperty = $this->resolvePropertyReflection($object, $propertyName);
-        $reflectionProperty->setAccessible(true);
 
         $reflectionProperty->setValue($object, $value);
     }
 
     private function createAccessibleMethodReflection(object $object, string $methodName): ReflectionMethod
     {
-        $reflectionMethod = new ReflectionMethod($object, $methodName);
-        $reflectionMethod->setAccessible(true);
-
-        return $reflectionMethod;
+        return new ReflectionMethod($object, $methodName);
     }
 
     private function resolvePropertyReflection(object $object, string $propertyName): ReflectionProperty