diff --git a/Doc/data/stable_abi.dat b/Doc/data/stable_abi.dat
index 2d4278c9d97c85..5936ce128bdf60 100644
--- a/Doc/data/stable_abi.dat
+++ b/Doc/data/stable_abi.dat
@@ -46,6 +46,7 @@ macro,PyBUF_STRIDES,3.11,,
 macro,PyBUF_WRITABLE,3.11,,
 macro,PyBUF_WRITE,3.11,,
 data,PyBaseObject_Type,3.2,,
+func,PyBool_Check,3.16,,
 func,PyBool_FromLong,3.2,,
 data,PyBool_Type,3.2,,
 func,PyBuffer_FillContiguousStrides,3.11,,
diff --git a/Include/boolobject.h b/Include/boolobject.h
index b56e2baecaa36c..0e7db5d47bf49c 100644
--- a/Include/boolobject.h
+++ b/Include/boolobject.h
@@ -9,7 +9,14 @@ extern "C" {
 
 // PyBool_Type is declared by object.h
 
-#define PyBool_Check(x) Py_IS_TYPE((x), &PyBool_Type)
+#if !defined(Py_LIMITED_API) || Py_LIMITED_API+0 >= _Py_PACK_VERSION(3, 16)
+PyAPI_FUNC(int) PyBool_Check(PyObject *x);
+#endif
+#if !defined(Py_LIMITED_API) || Py_LIMITED_API+0 < _Py_PACK_VERSION(3, 16)
+#  define PyBool_Check(x) Py_IS_TYPE((x), &PyBool_Type)
+#else
+#  define PyBool_Check(x) PyBool_Check(_PyObject_CAST(x))
+#endif
 
 /* Py_False and Py_True are the only two bools in existence. */
 
diff --git a/Lib/test/test_stable_abi_ctypes.py b/Lib/test/test_stable_abi_ctypes.py
index ac5c4296c663d0..f560f5cd472a79 100644
--- a/Lib/test/test_stable_abi_ctypes.py
+++ b/Lib/test/test_stable_abi_ctypes.py
@@ -55,6 +55,7 @@ def test_windows_feature_macros(self):
     "PyArg_VaParseTupleAndKeywords",
     "PyArg_ValidateKeywordArguments",
     "PyBaseObject_Type",
+    "PyBool_Check",
     "PyBool_FromLong",
     "PyBool_Type",
     "PyBuffer_FillContiguousStrides",
diff --git a/Misc/NEWS.d/next/C_API/2026-05-08-16-36-36.gh-issue-000000.2F1XTY.rst b/Misc/NEWS.d/next/C_API/2026-05-08-16-36-36.gh-issue-000000.2F1XTY.rst
new file mode 100644
index 00000000000000..070ac30961d7bd
--- /dev/null
+++ b/Misc/NEWS.d/next/C_API/2026-05-08-16-36-36.gh-issue-000000.2F1XTY.rst
@@ -0,0 +1,2 @@
+In the Limited C API 3.16 and newer, :c:func:`PyBool_Check` is now available
+as a function instead of a macro.
diff --git a/Misc/stable_abi.toml b/Misc/stable_abi.toml
index 8fd7aba09241e6..7f61fffae4bc3b 100644
--- a/Misc/stable_abi.toml
+++ b/Misc/stable_abi.toml
@@ -2831,3 +2831,6 @@
 
 [function.PyObject_CallFinalizerFromDealloc]
     added = '3.15'
+
+[function.PyBool_Check]
+    added = '3.16'
diff --git a/Objects/boolobject.c b/Objects/boolobject.c
index b694691ae4d0d1..7b090ff6b47df8 100644
--- a/Objects/boolobject.c
+++ b/Objects/boolobject.c
@@ -225,3 +225,8 @@ struct _longobject _Py_TrueStruct = {
         { 1 }
     }
 };
+
+// Implementations for the Stable ABI
+
+#undef PyBool_Check
+int PyBool_Check(PyObject *x) { return Py_IS_TYPE(x, &PyBool_Type); }
diff --git a/PC/python3dll.c b/PC/python3dll.c
index e0be9d65a93cda..f01f445e46f357 100755
--- a/PC/python3dll.c
+++ b/PC/python3dll.c
@@ -105,6 +105,7 @@ EXPORT_FUNC(PyArg_UnpackTuple)
 EXPORT_FUNC(PyArg_ValidateKeywordArguments)
 EXPORT_FUNC(PyArg_VaParse)
 EXPORT_FUNC(PyArg_VaParseTupleAndKeywords)
+EXPORT_FUNC(PyBool_Check)
 EXPORT_FUNC(PyBool_FromLong)
 EXPORT_FUNC(PyBuffer_FillContiguousStrides)
 EXPORT_FUNC(PyBuffer_FillInfo)