diff --git a/python/pyspark/sql/tests/test_python_datasource.py b/python/pyspark/sql/tests/test_python_datasource.py
index cfedf1cf075b..ee2d0ac60d90 100644
--- a/python/pyspark/sql/tests/test_python_datasource.py
+++ b/python/pyspark/sql/tests/test_python_datasource.py
@@ -258,7 +258,6 @@ def test_read_with_invalid_return_row_type(self):
         with self.assertRaisesRegex(PythonException, "DATA_SOURCE_INVALID_RETURN_TYPE"):
             df.collect()
 
-    @unittest.skipIf(sys.version_info > (3, 13), "SPARK-54065")
     def test_in_memory_data_source(self):
         class InMemDataSourceReader(DataSourceReader):
             DEFAULT_NUM_PARTITIONS: int = 3