From 2160a6a1428c04b8a2753ab0b49e6d15aaac78aa Mon Sep 17 00:00:00 2001 From: Cameron Davidson-Pilon Date: Mon, 23 Jun 2025 15:12:37 -0400 Subject: [PATCH] Fix spelling and grammar errors in docs --- .../02-Secondary level education/01-control-testing.md | 2 +- .../02-Secondary level education/caffeine-inhibition.md | 2 +- .../low-vs-high-conc-media.md | 4 ++-- .../02-Secondary level education/salt-stress-on-yeast.md | 4 ++-- .../02-Secondary level education/yeast-in-ethanol.md | 8 ++++---- .../directed-evolution-salt-tolerance.md | 2 +- .../00_pioreactor_40ml/04-40ml-faceplate-all-together.md | 4 ++-- .../04-v11-faceplate-all-together.md | 4 ++-- .../04-pioreactor-reassembly.md | 4 ++-- user-guide/99-common-questions.mdx | 4 ++-- 10 files changed, 19 insertions(+), 19 deletions(-) diff --git a/experiments/02-Secondary level education/01-control-testing.md b/experiments/02-Secondary level education/01-control-testing.md index abd1d7f87..6efa94aa5 100644 --- a/experiments/02-Secondary level education/01-control-testing.md +++ b/experiments/02-Secondary level education/01-control-testing.md @@ -42,7 +42,7 @@ Here are graphs we generated though our A/A test: ![](/img/experiments/aa-test-results.png) -These kinds of tests make it easy to identify any unusual activity occuring in a specific Pioreactor (i.e, if it is missing an LED, temperature malfunctions, etc). In our case, though, both ran almost identically! Thus, we conclude that reliable comparisons can be drawn between these Pioreactors. +These kinds of tests make it easy to identify any unusual activity occurring in a specific Pioreactor (i.e, if it is missing an LED, temperature malfunctions, etc). In our case, though, both ran almost identically! Thus, we conclude that reliable comparisons can be drawn between these Pioreactors. ## Detailed procedure diff --git a/experiments/02-Secondary level education/caffeine-inhibition.md b/experiments/02-Secondary level education/caffeine-inhibition.md index 3e0d939c8..fd66c466f 100644 --- a/experiments/02-Secondary level education/caffeine-inhibition.md +++ b/experiments/02-Secondary level education/caffeine-inhibition.md @@ -37,7 +37,7 @@ We used two Pioreactors and two vials containing 15 mL of YPD media. We added 1 ### Results -The growth rate chart clearly demonstrates an inhibition of growth occuring in the sample containing tea: +The growth rate chart clearly demonstrates an inhibition of growth occurring in the sample containing tea: ![](/img/experiments/tea2-exp-results.png) diff --git a/experiments/02-Secondary level education/low-vs-high-conc-media.md b/experiments/02-Secondary level education/low-vs-high-conc-media.md index 064faab24..73dcfec80 100644 --- a/experiments/02-Secondary level education/low-vs-high-conc-media.md +++ b/experiments/02-Secondary level education/low-vs-high-conc-media.md @@ -28,7 +28,7 @@ Conduct a simple comparison of yeast growth between a low percent and high perce The most accessible introductory experiment you can conduct with basic supplies is comparing concentrations of media. In this particular example, we will use YPD media and grow yeast cultures, but any media/culture you have readily available will work! -YPD is a common complete media used to propagate yeast cultures, containing peptone and sugars nexcessary for yeast growth. Now consider creating two YPD media compositions: one with 1% YPD w/v, and one with 5% YPD w/v. We can easily infer that a culture grown in 5% YPD will flourish more than one grown in 1% — but let's expore our hypothesis further! +YPD is a common complete media used to propagate yeast cultures, containing peptone and sugars necessary for yeast growth. Now consider creating two YPD media compositions: one with 1% YPD w/v, and one with 5% YPD w/v. We can easily infer that a culture grown in 5% YPD will flourish more than one grown in 1% — but let's explore our hypothesis further! Discuss with your students the following ideas: * How would both cultures start off? Will one have a faster rate than another? Why? @@ -51,7 +51,7 @@ $$ Using the same logic, 5% YPD was created by adding 0.75 grams to 15 mL. -We inoculated both with 100 uL of a yeast slurry using a micropipette. If this is unavailable, a dropped will work as well! +We inoculated both with 100 uL of a yeast slurry using a micropipette. If this is unavailable, a dropper will work as well! ### Results diff --git a/experiments/02-Secondary level education/salt-stress-on-yeast.md b/experiments/02-Secondary level education/salt-stress-on-yeast.md index c642cf41c..71592e108 100644 --- a/experiments/02-Secondary level education/salt-stress-on-yeast.md +++ b/experiments/02-Secondary level education/salt-stress-on-yeast.md @@ -54,7 +54,7 @@ $$ \text{mass} = 0.45 \text{ g} $$ Therefore, **0.45 grams** of table salt is needed for 3% w/v of salt. -To each vial, 0g, 0.225g, 0.45g, 0.675g and 0.9g of table salt were added, repectively. They were then innoculated with one drop of rehydrated active yeast (0.500 g of dry yeast in a 15 mL YPD broth vial). The _Temperature automation_ was set on _Thermostat_ at 32.5°C. +To each vial, 0g, 0.225g, 0.45g, 0.675g and 0.9g of table salt were added, respectively. They were then inoculated with one drop of rehydrated active yeast (0.500 g of dry yeast in a 15 mL YPD broth vial). The _Temperature automation_ was set on _Thermostat_ at 32.5°C. ### Results @@ -72,7 +72,7 @@ We can summarize our results in the following table to highlight our data: |4.5%|0.29|17 hours| |6%|0.22|24 hours| -The highest growth was recorded in the vial at 0% salt w/v (no salt stress), at approximately **0.78 h⁻¹**. The phases of growth (lag, log phase) occured quickly and the stationary phase was reached in the smallest time frame of about **8 hours**. +The highest growth was recorded in the vial at 0% salt w/v (no salt stress), at approximately **0.78 h⁻¹**. The phases of growth (lag, log phase) occurred quickly and the stationary phase was reached in the smallest time frame of about **8 hours**. As the salt percentage increased, the peak growth rate decreased and the time to reach the stationary phase was extended. These time increases are non-linear; between 0% and 1.5% salt, the stationary phase was reached within an increment of half an hour — however 4.5% and 6% salt had a large increment of 7 hours (from 17 to 24 hours). diff --git a/experiments/02-Secondary level education/yeast-in-ethanol.md b/experiments/02-Secondary level education/yeast-in-ethanol.md index bbf1c7e2b..04a82913e 100644 --- a/experiments/02-Secondary level education/yeast-in-ethanol.md +++ b/experiments/02-Secondary level education/yeast-in-ethanol.md @@ -4,7 +4,7 @@ slug: /yeast-in-ethanol tags: - Yeast - Ethanol - - Seconday education + - Secondary education --- import AssemblyInstructionBlock from '@site/src/components/AssemblyInstructionBlock'; @@ -32,7 +32,7 @@ While we have found many applications for yeast products, yeasts themselves see We know that most yeasts can tolerate an alcohol concentration of 10-15% before dying. Now, let's choose some alcohol concentrations and explore this inhibition using our Pioreactor! -We've included two methods to calculate ethanol precentages; teachers can prepare this beforehand or allow their students to calculate it themselves. +We've included two methods to calculate ethanol percentages; teachers can prepare this beforehand or allow their students to calculate it themselves. ## Experiment @@ -78,7 +78,7 @@ So the composition to create 2% ethanol media is: * 0.66 mL distilled water (1 mL - 0.34 mL) :::tip -If you don't have a small enough resolution on your measuring tools, you can increase the total amount of ethanol dilution. For example, instead of making 1 mL diluted ethanol, we used **3.4 mL** of 95% ethanol and **6.6 mL** of distilled water measured in a graduated cylindar to create a total 10 mL diluted ethanol. From that, we mixed well and added 1 mL into our vial. +If you don't have a small enough resolution on your measuring tools, you can increase the total amount of ethanol dilution. For example, instead of making 1 mL diluted ethanol, we used **3.4 mL** of 95% ethanol and **6.6 mL** of distilled water measured in a graduated cylinder to create a total 10 mL diluted ethanol. From that, we mixed well and added 1 mL into our vial. ::: We repeated this for 4% and 6% ethanol concentrations. @@ -138,7 +138,7 @@ Plenty of yeast strains are available at your local breweries. You can do the sa 2. Prepare 1 mL of each ethanol concentration you would like to use. * Detailed calculations are found in the example above. Aim for an end ethanol concentration between 0-10%. 3. Evenly divide the YPD stock into the vials you’d like to use (ie. 15 mL in each vial). -4. Add the mililitre of ethanol solution into the respective vial. Mix with gentle shaking. +4. Add the millilitre of ethanol solution into the respective vial. Mix with gentle shaking. 5. Inoculate the stock with a very small amount of baker's yeast using best practices to avoid other contamination. Wait for the yeast granules to dissolve, aided by gentle rocking or stirring. * Alternatively, a yeast stock solution can be made by diluting a small amount of yeast in 15 mL of YPD stock, then 1 drop of this stock solution can be added to your vials. 6. Wipe the vials and place them in the Pioreactors. diff --git a/experiments/04-Research and beyond/directed-evolution-salt-tolerance.md b/experiments/04-Research and beyond/directed-evolution-salt-tolerance.md index a8b6da41a..c05d67d5d 100644 --- a/experiments/04-Research and beyond/directed-evolution-salt-tolerance.md +++ b/experiments/04-Research and beyond/directed-evolution-salt-tolerance.md @@ -54,7 +54,7 @@ The following normalized OD graph resulted from our turbidostat using salted med ![](/img/experiments/turbidostat/salt_turbidostat.png) -A maximum growth rate of **0.6 h⁻¹** was reached. In contrast, when you look back at our [secondary level salt experiment](/experiments/salt-stress-on-yeast), the 4.5% w/v salt vial only reached **0.29 h⁻¹** before the stationary phase occured. This is because our previous experiment **selected** for salt tolerant yeast, but the culture reached stationary phase before any **long term adaptation** could be observed. +A maximum growth rate of **0.6 h⁻¹** was reached. In contrast, when you look back at our [secondary level salt experiment](/experiments/salt-stress-on-yeast), the 4.5% w/v salt vial only reached **0.29 h⁻¹** before the stationary phase occurred. This is because our previous experiment **selected** for salt tolerant yeast, but the culture reached stationary phase before any **long term adaptation** could be observed. Using the turbidostat system to supply new nutrients and keep the population low, we were able to _double_ the maximum growth rate! diff --git a/user-guide/01-getting-started/01-assembly_guides/00_pioreactor_40ml/04-40ml-faceplate-all-together.md b/user-guide/01-getting-started/01-assembly_guides/00_pioreactor_40ml/04-40ml-faceplate-all-together.md index be95b3824..d62d6cdf1 100644 --- a/user-guide/01-getting-started/01-assembly_guides/00_pioreactor_40ml/04-40ml-faceplate-all-together.md +++ b/user-guide/01-getting-started/01-assembly_guides/00_pioreactor_40ml/04-40ml-faceplate-all-together.md @@ -59,7 +59,7 @@ The top faceplate comes with a button extension to provide easier access to push 1. Flip over the assembly. 2. Insert the 10mm screw into the hole under the button extension. -3. Place one finger behind the extension and apply torque with the other hand until the screw is secure in the hole. This may require some force. Once established, you can use a screwdriver to finish screwing it in under you feel sufficient resistance. Don't over-tighten! +3. Place one finger behind the extension and apply torque with the other hand until the screw is secure in the hole. This may require some force. Once established, you can use a screwdriver to finish screwing it in until you feel sufficient resistance. Don't over-tighten! @@ -131,7 +131,7 @@ The top faceplate comes with a button extension to provide easier access to push 1. Flip over the assembly. 2. Insert the 10mm screw into the hole under the button extension. -3. Place one finger behind the extension and apply torque with the other hand until the screw is secure in the hole. This may require some force. Once established, you can use a screwdriver to finish screwing it in under you feel sufficient resistance. Don't over-tighten! +3. Place one finger behind the extension and apply torque with the other hand until the screw is secure in the hole. This may require some force. Once established, you can use a screwdriver to finish screwing it in until you feel sufficient resistance. Don't over-tighten! diff --git a/user-guide/01-getting-started/01-assembly_guides/01_pioreactor_20ml_v1.1/04-v11-faceplate-all-together.md b/user-guide/01-getting-started/01-assembly_guides/01_pioreactor_20ml_v1.1/04-v11-faceplate-all-together.md index cea4c0638..53fd0377a 100644 --- a/user-guide/01-getting-started/01-assembly_guides/01_pioreactor_20ml_v1.1/04-v11-faceplate-all-together.md +++ b/user-guide/01-getting-started/01-assembly_guides/01_pioreactor_20ml_v1.1/04-v11-faceplate-all-together.md @@ -59,7 +59,7 @@ The top faceplate comes with a button extension to provide easier access to push 1. Flip over the assembly. 2. Insert the 10mm screw into the hole under the button extension. -3. Place one finger behind the extension and apply torque with the other hand until the screw is secure in the hole. This may require some force. Once established, you can use a screwdriver to finish screwing it in under you feel sufficient resistance. Don't over-tighten! +3. Place one finger behind the extension and apply torque with the other hand until the screw is secure in the hole. This may require some force. Once established, you can use a screwdriver to finish screwing it in until you feel sufficient resistance. Don't over-tighten! @@ -131,7 +131,7 @@ The top faceplate comes with a button extension to provide easier access to push 1. Flip over the assembly. 2. Insert the 10mm screw into the hole under the button extension. -3. Place one finger behind the extension and apply torque with the other hand until the screw is secure in the hole. This may require some force. Once established, you can use a screwdriver to finish screwing it in under you feel sufficient resistance. Don't over-tighten! +3. Place one finger behind the extension and apply torque with the other hand until the screw is secure in the hole. This may require some force. Once established, you can use a screwdriver to finish screwing it in until you feel sufficient resistance. Don't over-tighten! diff --git a/user-guide/01-getting-started/01-assembly_guides/02_pioreactor_20ml_v1.0_to_v1.1/04-pioreactor-reassembly.md b/user-guide/01-getting-started/01-assembly_guides/02_pioreactor_20ml_v1.0_to_v1.1/04-pioreactor-reassembly.md index d94f63ce9..8da43e936 100644 --- a/user-guide/01-getting-started/01-assembly_guides/02_pioreactor_20ml_v1.0_to_v1.1/04-pioreactor-reassembly.md +++ b/user-guide/01-getting-started/01-assembly_guides/02_pioreactor_20ml_v1.0_to_v1.1/04-pioreactor-reassembly.md @@ -59,7 +59,7 @@ The top faceplate comes with a button extension to provide easier access to push 1. Flip over the assembly. 2. Insert the 10mm screw into the hole under the button extension. -3. Place one finger behind the extension and apply torque with the other hand until the screw is secure in the hole. This may require some force. Once established, you can use a screwdriver to finish screwing it in under you feel sufficient resistance. Don't over-tighten! +3. Place one finger behind the extension and apply torque with the other hand until the screw is secure in the hole. This may require some force. Once established, you can use a screwdriver to finish screwing it in until you feel sufficient resistance. Don't over-tighten! @@ -131,7 +131,7 @@ The top faceplate comes with a button extension to provide easier access to push 1. Flip over the assembly. 2. Insert the 10mm screw into the hole under the button extension. -3. Place one finger behind the extension and apply torque with the other hand until the screw is secure in the hole. This may require some force. Once established, you can use a screwdriver to finish screwing it in under you feel sufficient resistance. Don't over-tighten! +3. Place one finger behind the extension and apply torque with the other hand until the screw is secure in the hole. This may require some force. Once established, you can use a screwdriver to finish screwing it in until you feel sufficient resistance. Don't over-tighten! diff --git a/user-guide/99-common-questions.mdx b/user-guide/99-common-questions.mdx index e28ab06d0..2189f2075 100644 --- a/user-guide/99-common-questions.mdx +++ b/user-guide/99-common-questions.mdx @@ -127,9 +127,9 @@ Stirring is performed with a micro stir bar inside the vial, controlled by a pai Of course! The maximum length of a stir bar is 20mm. Keep the height low as to not interfere with the optics. (The provided stir bar is 3mm high). Try using other stir bar shapes / designs! -### The magnets rubs against the plastic screws above them, causing the stirring performance to be degraded / stop. How do I fix this? +### The magnets rub against the plastic screws above them, causing the stirring performance to be degraded / stop. How do I fix this? -Dis-attach the top faceplate. Under the faceplate, slightly unscrew each of the four metal screws. This will add more space between the magnets and the plastic screws. +Detach the top faceplate. Under the faceplate, slightly unscrew each of the four metal screws. This will add more space between the magnets and the plastic screws. ### How can I increase the strength of the magnetic force applied to the stirbar?