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Update defaults for TCR and update repseqio dependencies
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singlecell/resources/chunks/SeuratPrototype.R

Lines changed: 1 addition & 1 deletion
Original file line numberDiff line numberDiff line change
@@ -71,7 +71,7 @@ for (datasetId in names(seuratObjects)) {
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if ('Saturation.RNA' %in% names(seuratObj@meta.data)) {
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meanSaturation.RNA <- mean(seuratObj$Saturation.RNA)
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if (!is.null(minSaturation) && meanSaturation.RNA < minSaturation) {
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addErrorMessage(paste0('Mean RNA saturation was: ', meanSaturation.RNA, ' for dataset: ', datasetId, ', below threshold of: ', minSaturation))
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addErrorMessage(paste0('Mean RNA saturation was: ', meanSaturation.RNA, ' for dataset: ', datasetId, ', below threshold of: ', minSaturation, ', total cells: ', ncol(seuratObj)))
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}
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metricData <- rbind(metricData, data.frame(dataId = datasetId, readsetId = datasetIdToReadset[[datasetId]], metricname = 'MeanSaturation.RNA', metricvalue = meanSaturation.RNA))

singlecell/src/org/labkey/singlecell/pipeline/singlecell/AppendTcr.java

Lines changed: 2 additions & 2 deletions
Original file line numberDiff line numberDiff line change
@@ -23,8 +23,8 @@ public Provider()
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super("AppendTcr", "Append TCR Data", "RDiscvr", "This uses Rdiscvr::DownloadAndAppendTcrClonotypes to append TCR data.", List.of(
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SeuratToolParameter.create("allowMissing", "Allow Missing Data", "If checked, an error will be thrown if any sample lacks TCR data", "checkbox", new JSONObject()
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{{
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put("checked", true);
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}}, true)
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put("checked", false);
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}}, false)
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), null, null);
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}
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