Skip to content

Commit ed8f96d

Browse files
committed
Tweak to logic for hashing barcode export
1 parent 9b8c21e commit ed8f96d

File tree

1 file changed

+4
-1
lines changed

1 file changed

+4
-1
lines changed

singlecell/resources/web/singlecell/panel/LibraryExportPanel.js

Lines changed: 4 additions & 1 deletion
Original file line numberDiff line numberDiff line change
@@ -888,7 +888,10 @@ Ext4.define('SingleCell.panel.LibraryExportPanel', {
888888
var tcrData = totalCells > 15000 ? 45 : 25;
889889
processType(readsetIds, rows, r, 'readsetId', 'GEX', 500, 0.01, 'G', null, false, gexData, runMap, totalCells);
890890
processType(readsetIds, rows, r, 'tcrReadsetId', 'TCR', 700, 0.01, 'T', null, false, tcrData, runMap, totalCells);
891-
processType(readsetIds, rows, r, 'hashingReadsetId', 'HTO', 182, 0.05, 'H', 'Cell hashing, 190bp amplicon. Please QC individually and pool in equal amounts per lane', true, 20, runMap, totalCells);
891+
892+
// NOTE: Dual index 10x is always presented in the right orientation, so only RC if single-indexed
893+
const hashingDoRC = !r.barcode3;
894+
processType(readsetIds, rows, r, 'hashingReadsetId', 'HTO', 182, 0.05, 'H', 'Cell hashing, 190bp amplicon. Please QC individually and pool in equal amounts per lane', hashingDoRC, 20, runMap, totalCells);
892895
processType(readsetIds, rows, r, 'citeseqReadsetId', 'CITE', 182, 0.05, 'C', 'CITE-Seq, 190bp amplicon. Please QC individually and pool in equal amounts per lane', false, 20, runMap, totalCells);
893896
}, this);
894897

0 commit comments

Comments
 (0)